Summary: RNAs are a diverse group of molecules that exist ubiquitously in nature. They may possess varying biological functions and can be utilised for varying therapeutic goals. The function of RNA is driven by its sequence. CRISPR sgRNA and mRNA vaccines consist of different sequences and act differently. CRISPR sgRNA binds to its target genome sequence and Cas9 protein in […]
The side effects of CRISPR / Cas9 and the current strategies to mitigate them Summary: The CRISPR/Cas9 system often not only cuts at the intended site of the genome, but also in other unwanted places, which are called off-targets; Off-target events are mainly due to sequence homology between sgRNA and genomic DNA other than the target one, as well as […]
DNA repair mechanisms as the key to engineering human genes by CRISPR/Cas9 Summary: In 2020, CRISPR/Cas9 technology achieved several breakthroughs that are revolutionizing the fields of food and medicine. CRISPR/Cas9 makes a break in DNA, triggering several cell repair mechanisms that researchers exploit to manipulate the genome of the organisms. The DNA repair system referred to as non-homologous end joining […]